3VCF

SSV1 integrase C-terminal catalytic domain (174-335aa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	89.051, 89.051, 147.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.110 - 2.700
R-factor	0.1892
Rwork	0.187
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.110	2.950
High resolution limit [Å]	2.700	2.700
Number of reflections	16941
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	289	0.056M NaH2PO4, 1.35M K2HPO4, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K