3VC8

Crystal structure of the C-terminal cytoplasmic domain of non-structural protein 4 from mouse hepatitis virus A59

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	86.862, 52.549, 53.873
Unit cell angles	90.00, 116.41, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.221
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.202
Number of reflections	14589
<I/σ(I)>		6.1
Completeness [%]	98.6	89.4
Redundancy		6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	1.0 L nsp4C at 3 mg/mL was added to the same volume of reservoir solution (10% PEG MME550, 0.1 M glycine, pH 9.0, and 0.01 M zinc sulfate), VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	9	291	1.0 L nsp4C at 3 mg/mL was added to the same volume of reservoir solution (10% PEG MME550, 0.1 M glycine, pH 9.0, and 0.01 M zinc sulfate), VAPOR DIFFUSION, HANGING DROP, temperature 291K