3VC7
Crystal Structure of a putative oxidoreductase from Sinorhizobium meliloti 1021
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 59.074, 119.301, 64.128 |
Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
Resolution | 32.322 - 2.231 |
R-factor | 0.1821 |
Rwork | 0.180 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3emk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.833 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 39837 | |
Completeness [%] | 99.7 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 200 mM MgCl2, 100 mM Tris, and 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |