3VBZ
Crystal structure of Taipoxin beta subunit isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91232 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.570, 36.480, 65.560 |
| Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
| Resolution | 33.330 - 1.760 |
| R-factor | 0.18443 |
| Rwork | 0.182 |
| R-free | 0.22674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ae7 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.002 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.330 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.066 | 0.278 |
| Number of reflections | 23192 | |
| <I/σ(I)> | 8.1 | 2.7 |
| Completeness [%] | 99.0 | 95.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M NaCl, 0.1 M HEPES Sodium salt, 1.0 M Sodium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
