3VBS
Crystal structure of human Enterovirus 71
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2011-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96860 |
Spacegroup name | I 2 3 |
Unit cell lengths | 599.800, 599.800, 599.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.980 - 3.000 |
R-factor | 0.273 |
Rwork | 0.273 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vbf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.680 | |
Number of reflections | 612286 | |
<I/σ(I)> | 1.2 | 0.5 |
Completeness [%] | 86.8 | 84.5 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG400, 0.2 M tri-Sodium Citrate, 0.1 M Tris.HCl pH 8.5 MIXED WITH VIRUS AND equilibrated againsT SALT reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 293K |