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3VBS

Crystal structure of human Enterovirus 71

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]293
Detector technologyPIXEL
Collection date2011-10-20
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.96860
Spacegroup nameI 2 3
Unit cell lengths599.800, 599.800, 599.800
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.980 - 3.000
R-factor0.273
Rwork0.273
R-free0.28500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3vbf
RMSD bond length0.009
RMSD bond angle1.500
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.110
High resolution limit [Å]3.0003.000
Rmerge0.680
Number of reflections612286
<I/σ(I)>1.20.5
Completeness [%]86.884.5
Redundancy2.42.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.529330% PEG400, 0.2 M tri-Sodium Citrate, 0.1 M Tris.HCl pH 8.5 MIXED WITH VIRUS AND equilibrated againsT SALT reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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