3VBO
Crystal structure of formaldehyde treated empty human Enterovirus 71 particle (cryo at 100K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9750 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 353.100, 353.100, 353.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.880 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STARTING MODEL 1BEV |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Number of reflections | 167672 | |
| <I/σ(I)> | 6 | 1.2 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 23.5 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200 mM Ammonium phosphate monobasic, 24% (v/v) Isopropanol and 100 mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
