3VB1

Crystal Structure of Anopholes gambiae odorant binding protein 20 in open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-12
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	34.923, 36.463, 92.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.650 - 2.000
R-factor	0.2348
Rwork	0.231
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v2l
RMSD bond length	0.014
RMSD bond angle	1.383
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	PHASER (1.3.3)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.900	33.900	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.046	0.026	0.198
Total number of observations		4113	4095
Number of reflections	9029
<I/σ(I)>	15.8	36.8	5.5
Completeness [%]	99.7	99.9	100
Redundancy	4.41	4.13	4.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.57	276	1.9 M ammonium sulfate, 0.2 M potassium sodium tartrate, 0.1 M sodium citrate, pH 5.57, VAPOR DIFFUSION, HANGING DROP, temperature 276K