3VAL
Structure of U2AF65 variant with BrU5C1 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.583, 137.081, 83.962 |
| Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.208 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g4b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.147 | 0.115 | 0.324 |
| <I/σ(I)> | 17.7 | ||
| Completeness [%] | 95.1 | 100 | 69.1 |
| Redundancy | 6.7 | 7.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
