3VAH
Structure of U2AF65 variant with BrU3C4 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.238, 37.304, 101.310 |
| Unit cell angles | 90.00, 125.61, 90.00 |
Refinement procedure
| Resolution | 41.170 - 2.500 |
| R-factor | 0.2035 |
| Rwork | 0.198 |
| R-free | 0.25920 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.114 | 0.068 | 0.435 |
| Number of reflections | 17610 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 99.3 | 97.8 | 99.7 |
| Redundancy | 4.6 | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
