3VA8
Crystal structure of enolase FG03645.1 (target EFI-502278) from Gibberella zeae PH-1 complexed with magnesium, formate and sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 135.669, 135.669, 164.246 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17251 |
| Rwork | 0.170 |
| R-free | 0.21665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tw9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.071 | 0.900 |
| Number of reflections | 42699 | |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 98.1 | 74.8 |
| Redundancy | 5.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 0.2M AMMONIUM SULFATE, 100MM SODIUM CACODYLATE, PH 6.5, 30% PEG 8000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
