3V9L
Crystal structure of mouse 1-pyrroline-5-carboxylate dehydrogenase complexed with NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-27 |
Detector | NOIR-1 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.888, 94.053, 132.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.647 - 1.502 |
R-factor | 0.1588 |
Rwork | 0.158 |
R-free | 0.17580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v9j |
RMSD bond length | 0.006 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.027 | 47.027 | 1.580 |
High resolution limit [Å] | 1.502 | 4.750 | 1.502 |
Rmerge | 0.020 | 0.399 | |
Total number of observations | 40342 | 161483 | |
Number of reflections | 168338 | ||
<I/σ(I)> | 25.3 | 75 | 4.9 |
Completeness [%] | 99.6 | 99.9 | 97.6 |
Redundancy | 7.3 | 7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | reservoir: 20-25% w/v PEG3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, cryoprotectant: 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |