3V9J

Crystal structure of mouse 1-pyrroline-5-carboxylate dehydrogenase complexed with sulfate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	85.228, 94.039, 132.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.420 - 1.299
R-factor	0.1333
Rwork	0.132
R-free	0.15960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v9g
RMSD bond length	0.005
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.299	3.530	1.299
Rmerge	0.055	0.030	0.502
Number of reflections	247337
<I/σ(I)>	12.6
Completeness [%]	94.9	98.5	86
Redundancy	3.7	4.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	reservoir: 20-25% w/v PEG3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, cryoprotectant: 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K