3V9G

Crystal structure of human 1-pyrroline-5-carboxylate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979181
Spacegroup name	P 65
Unit cell lengths	150.717, 150.717, 191.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.665 - 2.500
R-factor	0.2048
Rwork	0.204
R-free	0.23850
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (autosol)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.075	0.055	0.539
Number of reflections	84960
<I/σ(I)>	14.6
Completeness [%]	99.7	97.7	100
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	reservoir: 22.5% PEG3350, 0.2 M ammonium sulfate, 0.1 M HEPES, cryoprotectant: 25% PEG3350, 0.2 M ammonium sulfate, 0.1 M HEPES, 25% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K