3V9E
Structure of the L499M mutant of the laccase from B.aclada
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9815 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.710, 113.560, 79.980 |
| Unit cell angles | 90.00, 108.74, 90.00 |
Refinement procedure
| Resolution | 28.550 - 1.700 |
| R-factor | 0.16514 |
| Rwork | 0.163 |
| R-free | 0.19981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sqr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.015 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 62839 | |
| Completeness [%] | 97.4 | 93.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 1.0 M lithium sulphate, 0.5 M ammonium sulphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
