3V9C

Type-2 Cu-depleted fungus laccase from Trametes hirsuta at low dose of ionization radiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-25
Detector	MAR CCD 165 mm
Wavelength(s)	0.81
Spacegroup name	P 21 21 21
Unit cell lengths	52.653, 77.051, 130.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.000
R-factor	0.16322
Rwork	0.161
R-free	0.20025
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fpx
RMSD bond length	0.012
RMSD bond angle	1.355
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.090
Number of reflections	35771
<I/σ(I)>	9.5
Completeness [%]	97.5	75
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Protein solution 44 mg/ml in 0.02M potassium phosphate, pH 6.0. Reservoir solution is 30% PEG 1500, 0.02M potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K