3V91
Structure of T82M glycogenin mutant truncated at residue 270 complexed with UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.430 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.210, 103.510, 119.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.370 - 2.000 |
| R-factor | 0.20775 |
| Rwork | 0.206 |
| R-free | 0.23582 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.370 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.308 |
| Number of reflections | 24729 | |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEGMME5000/0.2M ammonium sulfate/sodium MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
