3V90
Structure of T82M glycogenin mutant truncated at residue 270
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.430 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.590, 102.790, 119.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.750 - 2.000 |
R-factor | 0.19502 |
Rwork | 0.193 |
R-free | 0.22377 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.125 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.750 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.161 |
Number of reflections | 24591 | |
<I/σ(I)> | 8.5 | 4.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEGMME5000/0.2M ammonium sulfate/sodium MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |