3V8Z
Structure of apo-glycogenin truncated at residue 270 complexed with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.430 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.940, 105.170, 120.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.740 - 2.200 |
| R-factor | 0.20077 |
| Rwork | 0.199 |
| R-free | 0.23825 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.740 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.303 |
| Number of reflections | 18783 | |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 1.0 M ammonium sulfate/0.1 M sodium phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
