3V8O
Human Filamin C Ig - like Domains 4 and 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.072270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.620, 91.350, 103.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.800 |
| R-factor | 0.20565 |
| Rwork | 0.200 |
| R-free | 0.25493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nqc |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.925 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.633 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 15138 | |
| Completeness [%] | 97.9 | 98 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6M Sodium Potassium Phosphate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
