3V8K

Crystal structure of Staphylococcus aureus biotin protein ligase in complex with biotin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	277
Wavelength(s)	1.51
Spacegroup name	P 42 21 2
Unit cell lengths	94.266, 94.266, 130.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 3.230
R-factor	0.20494
Rwork	0.202
R-free	0.26796
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.314
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.800
High resolution limit [Å]	3.230
Number of reflections	9332

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K