3V8J

Crystal structure of Staphylococcus aureus biotin protein ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	277
Wavelength(s)	1.51
Spacegroup name	P 1 21 1
Unit cell lengths	50.132, 51.401, 67.584
Unit cell angles	90.00, 108.00, 90.00

Refinement procedure

Resolution	34.956 - 2.100
R-factor	0.2015
Rwork	0.199
R-free	0.25740
RMSD bond length	0.008
RMSD bond angle	1.182
Refinement software	PHENIX (phenix.refine: 1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.960
High resolution limit [Å]	2.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K