3V7J

Co-crystal structure of Wild Type Rat polymerase beta: Enzyme-DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.97907
Spacegroup name	P 21 21 21
Unit cell lengths	56.779, 77.114, 116.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.018 - 2.250
R-factor	0.2129
Rwork	0.211
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.325
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7.2_869)
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.018	2.320
High resolution limit [Å]	2.250	2.250
Number of reflections	25465
<I/σ(I)>		2.03
Completeness [%]	95.7	94.7
Redundancy	4.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	296	PEG 3350, NaCl, Glycerol, Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K