3V78
Crystal Structure of Transcriptional Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.098, 99.098, 66.478 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.728 - 2.299 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t6n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.057 | 0.031 | 0.364 |
| Number of reflections | 32337 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.6 | 99.6 | 100 |
| Redundancy | 3.2 | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris, PEG 4000, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
