3V77
Crystal structure of a putative fumarylacetoacetate isomerase/hydrolase from Oleispira antarctica
Replaces: 3L53Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-22 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 158.265, 161.386, 114.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.510 - 2.100 |
| R-factor | 0.20156 |
| Rwork | 0.199 |
| R-free | 0.25592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gtt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 97790 | |
| <I/σ(I)> | 20.88 | 1.76 |
| Completeness [%] | 98.7 | 83.5 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 1.2 M DI-AMMONIUM TARTRATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH 4.6, 10 MM ZINC SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
