3V74
crystal structure of FBF-2 in complex with gld-1 FBEa13 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-17 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.465, 98.465, 107.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.010 - 2.300 |
| Rwork | 0.177 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k5y |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.011 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.094 | 0.650 |
| Number of reflections | 26381 | |
| <I/σ(I)> | 17.7 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% [w/v] polyethylene glycol 6000, 5% [v/v] (+/-)-2-methyl-2,4-pentanediol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
