3V71
Crystal structure of PUF-6 in complex with 5BE13 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.806, 87.836, 129.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.902 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k5y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.073 | 0.540 |
| Number of reflections | 17479 | |
| <I/σ(I)> | 16.6 | 2.4 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% (w/v) PEG3350, 0.1M succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
