Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97948 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.348, 81.348, 178.934 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.822 - 2.206 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished model of GIMAP4. Related to PDB entry 3LXX. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.098 | 0.065 | 0.422 |
| Number of reflections | 21552 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 97.1 | 97.8 | 75.6 |
| Redundancy | 4.1 | 4.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 25% w/v PEG3350, 0.2M sodium chloride, 0.1M HEPES. GDP and 1:100 dispase were also added., pH 7.5, vapor diffusion, temperature 293K |
