3V6G
Crystal Structure of Transcriptional Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.396, 119.924, 30.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.625 - 1.821 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.24710 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.900 |
| High resolution limit [Å] | 1.820 | 3.940 | 1.820 |
| Rmerge | 0.049 | 0.027 | 0.398 |
| Number of reflections | 31246 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 98.4 | 99.2 | 96 |
| Redundancy | 2.9 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Hepes, PEG 4000, MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
