3V6D
Crystal structure of HIV-1 reverse transcriptase (RT) cross-linked with AZT-terminated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.476, 133.171, 139.904 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 44.300 - 2.705 |
| R-factor | 0.2341 |
| Rwork | 0.233 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.089 | 0.051 | 0.625 |
| Number of reflections | 86184 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 98.4 | 91 | 97.2 |
| Redundancy | 3.9 | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 277 | PEG 8000, ammonium sulfate, sucrose, glycerol, magnesium chloride, pH 6.8, VAPOR DIFFUSION, temperature 277K |
