3V6A

Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	46.376, 88.615, 136.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.21437
Rwork	0.213
R-free	0.25013
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0r
RMSD bond length	0.011
RMSD bond angle	1.337
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	17022
Completeness [%]	99.3	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	10% PEG 6000, 100mM bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K