3V68
Crystal structure of Pyrococcus furiosus PF2050, a member of DUF2666 family protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.97910, 0.97924, 0.9600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.722, 66.531, 46.372 |
| Unit cell angles | 90.00, 96.56, 90.00 |
Refinement procedure
| Resolution | 46.070 - 1.560 |
| R-factor | 0.19909 |
| Rwork | 0.198 |
| R-free | 0.22493 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.959 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.560 |
| Number of reflections | 32515 |
| Completeness [%] | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.02M calcium chloride dehydrate, 0.1M sodium acetate trihydrate, 30% 2-methyl-2,4-pentanediol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
