3V5Y

Structure of FBXL5 hemerythrin domain, P2(1) cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-06
Detector	ADSC Q315
Wavelength(s)	0.97188
Spacegroup name	P 1 21 1
Unit cell lengths	76.257, 54.373, 78.215
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	29.307 - 2.100
R-factor	0.2009
Rwork	0.197
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v5x
RMSD bond length	0.017
RMSD bond angle	1.403
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.063	0.028	0.597
Number of reflections	37291
<I/σ(I)>	9.4
Completeness [%]	97.5	91.6	98.8
Redundancy	2.7	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	25% PEG 3350, 0.1 M HEPES, 20% ethylene glycol, pH 7.4, vapor diffusion, hanging drop, temperature 293K