3V5N
The crystal structure of oxidoreductase from Sinorhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.196, 77.725, 107.733 |
| Unit cell angles | 90.00, 113.83, 90.00 |
Refinement procedure
| Resolution | 49.275 - 2.802 |
| R-factor | 0.1881 |
| Rwork | 0.185 |
| R-free | 0.25210 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.128 | 0.501 |
| Number of reflections | 40062 | |
| <I/σ(I)> | 9.7 | 2.3 |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M L-proline, 0.1 M HEPES pH 7.5, 10% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
