3V5B
Structure of Coil 2b of human lamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97690 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.020, 90.020, 74.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.490 - 3.000 |
| R-factor | 0.2864 |
| Rwork | 0.280 |
| R-free | 0.32600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 74.700 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 3889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 30% 2-methyl-2,4-pentanediol, 0.1 M Hepes pH 7.5, 0.5 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP |
