3V4U
Structure of a monoclonal antibody complexed with its MHC-I antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.140, 40.643, 59.069 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 41.670 - 1.640 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.670 | 1.720 |
| High resolution limit [Å] | 1.640 | 1.680 |
| Rmerge | 0.063 | |
| Number of reflections | 55324 | |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 99.8 | 88.4 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M LiSO4, 0.1M Tris, 30%(w/v)PEG 3000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
