3V4R
Crystal structure of a UvrB dimer-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.940, 100.310, 163.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 3.250 |
R-factor | 0.1817 |
Rwork | 0.180 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Protein co-ordinates from 2D7D |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.910 | 3.330 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.149 | 0.761 |
<I/σ(I)> | 9.3 | 2.72 |
Completeness [%] | 99.4 | 100 |
Redundancy | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 289 | Crystals were grown from 0.1M MES ph 6.5, 12% PEG 20,0000, Microbatch, temperature 289K |