3V4E
Crystal Structure of the galactoside O-acetyltransferase in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.424, 53.461, 53.477 |
| Unit cell angles | 89.75, 89.78, 89.94 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.18252 |
| Rwork | 0.180 |
| R-free | 0.22215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.034 | 0.075 |
| Number of reflections | 39809 | |
| <I/σ(I)> | 32.7 | 10.6 |
| Completeness [%] | 92.5 | 57.2 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M NA DIH PHOSPHATE, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
