3V3Y
Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-11-11 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.750, 139.750, 185.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.941 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e6d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.742 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.600 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.185 | 0.626 |
| Number of reflections | 51787 | |
| <I/σ(I)> | 6.11 | 1.7 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 3.75 | 3.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 3.5% 1,2,3 -heptanetriol, 2% dioxane, 0.1% LDAO, 1M potassium phosphate , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
