3V3C
Crystal Structure of Chloroplast ATP synthase c-ring from Pisum sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.130, 102.380, 122.430 |
| Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
| Resolution | 28.889 - 3.402 |
| R-factor | 0.3006 |
| Rwork | 0.299 |
| R-free | 0.32150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.320 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.167 | 0.033 |
| Number of reflections | 23125 | |
| <I/σ(I)> | 6.3 | 27.1 |
| Completeness [%] | 23.4 | |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100mM Na-acetate (pH 4.5), 50mM MgCl2, 50mM NaCl, 10mM Yttrium chloride and 14-24% PEG 550 Mono, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
