3V33
Crystal structure of MCPIP1 conserved domain with zinc-finger motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.531, 113.531, 78.016 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.259 - 2.005 |
R-factor | 0.1918 |
Rwork | 0.190 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.260 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | 0.567 |
Number of reflections | 30650 | |
<I/σ(I)> | 26.2 | 1.35 |
Completeness [%] | 78.0 | 33.4 |
Redundancy | 5.8 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 4% Tacsimate, 6% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |