3V2T
Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-25 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.112, 60.382, 38.473 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 25.047 - 1.800 |
| R-factor | 0.167 |
| Rwork | 0.161 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.541 |
| Data reduction software | APEX |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.047 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.022 | 0.156 |
| Number of reflections | 13205 | |
| <I/σ(I)> | 33.6 | 6.09 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 4.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
