3V21
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGT |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-01 |
| Detector | MAR555 |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.880, 115.311, 130.243 |
| Unit cell angles | 90.00, 112.50, 90.00 |
Refinement procedure
| Resolution | 65.090 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.230 |
| R-free | 0.26700 |
| Structure solution method | MR |
| Starting model (for MR) | 1knv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.288 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.094 | 65.090 | 2.850 |
| High resolution limit [Å] | 2.326 | 8.540 | 2.700 |
| Rmerge | 0.061 | 0.027 | 0.388 |
| Total number of observations | 11234 | 53541 | |
| Number of reflections | 80299 | ||
| <I/σ(I)> | 20.3 | 18.3 | 1.9 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 4.6 | 4.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 0.1 M Bis-Tris, 0.5% polyvinylpyrrolidone and 16% of PEG400, VAPOR DIFFUSION, temperature 293K, pH 5.5 |
