3V1Z
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.008, 83.632, 123.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.140 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Starting model (for MR) | 1knv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.140 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Rmerge | 0.067 | 0.307 |
| Number of reflections | 33372 | |
| <I/σ(I)> | 16.7 | 2.5 |
| Completeness [%] | 99.1 | 95.2 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | 100mM Na-acetate pH 4.25-5.5, 10mM CaCl2 or CaAc2, 4-8% (w/v) of PEG8000, temperature 293K |
