3V1Q

Crystal structures of the reverse transcriptase-associated ribonuclease H domain of xenotropic murine leukemia-virus related virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 41
Unit cell lengths	37.530, 37.530, 99.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.443 - 2.000
R-factor	0.1715
Rwork	0.169
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.000	2.000
Rmerge	0.146
Number of reflections	9361
<I/σ(I)>		1.71
Completeness [%]	99.7	99.7
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	40% PEG1500, 5 mM magnesium chloride, 100 mM sodium citrate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K