3V18
Structure of the Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2011-02-09 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.352, 58.001, 61.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.970 - 2.340 |
R-factor | 0.18554 |
Rwork | 0.182 |
R-free | 0.25669 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.618 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.340 | 2.350 |
Number of reflections | 13395 | |
Completeness [%] | 98.4 | 85 |
Redundancy | 6.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 4000, 10% isopropanol, 0.10M Hepes , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |