3V03
Crystal structure of Bovine Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.661, 45.096, 142.408 |
| Unit cell angles | 90.00, 114.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.20517 |
| Rwork | 0.202 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v08 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 (MOLREP) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.069 | 0.626 |
| Number of reflections | 35162 | |
| <I/σ(I)> | 29.1 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2 M Ca acetate, 20% PEG3350, 0.1 M Tris HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
