3UZO
Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.881, 75.980, 79.123 |
| Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2COJ |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.190 |
| Number of reflections | 42767 | |
| <I/σ(I)> | 26.7 | 8.61 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2M Na Chloride, 25%(w/v) polyethylene glycol 3350, 0.1M Bis-Tris, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.4 |
