3UZD
Crystal structure of 14-3-3 GAMMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 60.511, 79.230, 122.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.480 - 1.860 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.526 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.480 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 5.050 | 1.860 |
| Rmerge | 0.050 | 0.023 | 0.845 |
| Number of reflections | 24650 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 98.3 | 99.2 | 96.1 |
| Redundancy | 7.1 | 6.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.3M Mg (OAc)2, 20% PEG3350, vapor diffusion, hanging drop, temperature 291K |
