3UZ5
Designed protein KE59 R13 3/11H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.476, 123.744, 45.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.999 - 1.900 |
| R-factor | 0.18733 |
| Rwork | 0.184 |
| R-free | 0.25083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyc |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.999 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.149 | 0.567 |
| Number of reflections | 43250 | |
| <I/σ(I)> | 11.6 | 2.5 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M potassium thiocyanate, 0.1 M Bis-Tris propane, pH 6.5, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
