3UZ0

Crystal Structure of SpoIIIAH and SpoIIQ Complex

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	62.370, 64.490, 176.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.400 - 2.820
R-factor	0.213
Rwork	0.208
R-free	0.27000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.400	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.147	0.832
Number of reflections	36350
<I/σ(I)>	12.9	2
Completeness [%]	99.9	100
Redundancy	7.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	2.8M Ammonium sulfate, 10mM Mg acetate, 50 mM MES, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K